Phosphinothricin acetyltransferase
WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from … Web2.2 Preparation of the Phosphinothricin Acetyltransferase Electrochemical Immunosensor Prior to modification, the GCE was polished with 1 μm, 0.3 µm, and 0.05 µm aluminum powder sequentially on polishing paper, with sonication in double-distilled water between each polishing step.
Phosphinothricin acetyltransferase
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WebProduct: phosphinothricin acetyltransferase. confers resistance to bialophos or phosphinothricin: BlpR 373 .. 924 = 552 bp 183 amino acids = 20.6 kDa. Product: phosphinothricin acetyltransferase. confers resistance to bialophos or … WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis.
WebMay 26, 2024 · Phosphinothricin acetyltransferase (PAT), the target protein (Kim et al. 2010) investigated in this study, is a typical herbicide-resistant protein coded by a bar gene isolated from Streptomyces hygroscopicus … WebApr 1, 2005 · DP23211 maize expresses an insect-specific dsRNA, an insect-active non-Bt protein, the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate …
WebNov 1, 1996 · The steps involved are catalyzed by the following enzymes: step 1, PPT N-acetyltransferase (N-ac-PPT); step 2, deaminases, including L-amino acid oxidase; step 3, transaminase. Step 4 presumably ... WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells …
WebApr 15, 2024 · Here we demonstrate that purified PpArsN1 exhibits phosphinothricin acetyltransferase activity (Table 1). The glutamine synthetase inhibitor L-MSO is a poorer substrate compared with AST (Fig. 1d ).
WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... granny\u0027s spices india llpWebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) tiafenacil in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions comprising said … chinti und parkerWeb小鼠半乳糖苷酶β(glβ)elisa试剂盒 产品性状：盒装液体。 小鼠半乳糖苷酶β(glβ)elisa试剂盒提供免费检测服务，我们将根据实验工作量和难易程度，双方协定实验周期，保质保量完成委托实验，只收取试剂盒的费用，其他辅助试剂全部免费。 granny\\u0027s spring tonicWebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase (PAT) and bialaphos resistance (bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application.Following the rapid development of genome editing technology, generation of … granny\u0027s spring tonicWebl -Phosphinothricin ( l -Pt)-resistant plants were constructed by introducing a modified phosphinothricin- N -acetyl-transferase gene ( pat) via Agrobacterium -mediated gene … chint kwh meterWebYour search returned 12 phosphinothricin acetyltransferase ELISA Kit across 1 supplier. Showing 1 of 1 suppliers (12 products total) > >> Select All. Select up to 5 products from … ch in tlWebPPT N-acetyltransferase. acetyl-CoA + phosphinothricin <=> CoA + H (+) + N-acetylphosphinothricin. The substrate phosphinothricin is used as a nonselective herbicide and is a potent inhibitor of EC 6.3.1.2, a key enzyme of nitrogen metabolism in plants. All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in ... chint limit switch